The MRPL24 Polyclonal Antibody (CAB4967) is a valuable tool for research involving MRPL24, a mitochondrial ribosomal protein that plays a crucial role in mitochondrial protein synthesis. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding to the MRPL24 protein, this antibody facilitates the detection and analysis of MRPL24 in various cell types, making it an ideal choice for studies in mitochondrial biology and related fields.MRPL24 is a key component of the mitochondrial ribosome, which is responsible for translating mitochondrial DNA-encoded proteins.
Dysfunction in mitochondrial protein synthesis can lead to a variety of diseases, including metabolic disorders and neurodegenerative conditions. By studying MRPL24, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for treatment.Overall, the MRPL24 Polyclonal Antibody (CAB4967) is a valuable tool for researchers interested in unraveling the complexities of mitochondrial protein synthesis and its implications for human health. Its high specificity and reliability make it a valuable asset for studies in biochemistry, molecular biology, and related fields.
Product Name:
MRPL24 Rabbit Polyclonal Antibody
SKU:
CAB4967
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human MRPL24 (NP_078816.2).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24). Sequence analysis identified two transcript variants that encode the same protein.
Purification Method:
Affinity purification
Gene ID:
79590
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL24 Rabbit pAb (CAB4967) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
