The MRPL20 Polyclonal Antibody (PACO22089) is a valuable tool for researchers studying mitochondrial ribosomal protein MRPL20. This antibody, produced in rabbits, is highly specific and reacts strongly with human samples, making it ideal for use in Western blot applications. By binding to the MRPL20 protein, this antibody allows for precise detection and analysis in various cell types, providing valuable insights into mitochondrial function and potential roles in disease development.MRPL20 is a crucial component of mitochondrial ribosomes, which are responsible for protein synthesis within the mitochondria. Dysregulation of mitochondrial protein synthesis has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic diseases.
Studying the function and regulation of MRPL20 can provide important insights into mitochondrial biology and its implications for human health.With its high specificity and reactivity with human samples, the MRPL20 Polyclonal Antibody (PACO22089) is a reliable tool for researchers investigating the role of MRPL20 in mitochondrial protein synthesis and its potential contributions to disease development. This antibody is a valuable asset for studies in molecular biology, biochemistry, and medical research.
Antibody Name:
MRPL20 Antibody (PACO22089)
Antibody SKU:
PACO22089
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from C-terminal of human MRPL20.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COLO cells, HUVEC cells and HeLa cells, using MRPL20 antibody.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q.
Synonyms:
39S ribosomal protein L20; mitochondrial [Precursor]; L20mt; MRP-L20; MRPL20
UniProt Protein Function:
MRPL20: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. [provided by RefSeq, Jul 2008]Protein type: MitochondrialChromosomal Location of Human Ortholog: 1p36.3-p36.2Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrionMolecular Function: protein binding; rRNA binding; structural constituent of ribosomeBiological Process: mitochondrial translation; organelle organization and biogenesis; ribosomal large subunit assembly and maintenance
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
