The MRPL14 Polyclonal Antibody (PACO22086) is a valuable tool for researchers studying MRPL14, a mitochondrial ribosomal protein important for protein synthesis within mitochondria. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the MRPL14 protein, this antibody allows for detection and analysis in a variety of cell types, making it ideal for research in molecular biology and mitochondrial function.MRPL14 is a key player in mitochondrial protein synthesis and is therefore essential for the proper functioning of these organelles.
Research on MRPL14 is important for understanding mitochondrial biology and its role in various diseases, including metabolic disorders and neurodegenerative diseases. By studying the expression and function of MRPL14, researchers can gain insights into mitochondrial dysfunction and potentially develop new therapeutic approaches targeting mitochondrial protein synthesis pathways.
Antibody Name:
MRPL14 Antibody (PACO22086)
Antibody SKU:
PACO22086
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human MRPL14.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, COLO cells and 293 cells, using MRPL14 antibody.
Background:
Forms part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1 intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation Probable.
Synonyms:
39S ribosomal protein L14; mitochondrial; L14mt; MRP-L14; MRP-L32
UniProt Protein Function:
MRPL14: Forms part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1 intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation (Probable). Belongs to the ribosomal protein L14P family.Protein type: TranslationChromosomal Location of Human Ortholog: 6p21.3Cellular Component: mitochondrial inner membrane; ribosomeMolecular Function: structural constituent of ribosomeBiological Process: mitochondrial translation; organelle organization and biogenesis
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3. [provided by RefSeq, Jul 2008]
