MRPL12 Rabbit Polyclonal Antibody (CAB8318)
- SKU:
- CAB8318
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Product Name: | MRPL12 Rabbit Polyclonal Antibody |
SKU: | CAB8318 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2). |
Sequence: | MLPA AARP LWGP CLGL RAAA FRLA RRQV PCVC AVRH MRSS GHQR CEAL AGAP LDNA PKEY PPKI QQLV QDIA SLTL LEIS DLNE LLKK TLKI QDVG LVPM GGVM SGAV PAAA AQEA VEED IPIA KERT HFTV RLTE AKPV DKVK LIKE IKNY IQGI NLVQ AKKL VESL PQEI KANV AKAE AEKI KAAL EAVG GTVV LE |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
Synonyms: | 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34; MRPL12 |
Positive Sample: | 293F,HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion. |
Calculated MW: | 21kDa |
Observed MW: | 21kDa |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
Purification Method: | Affinity purification |
Gene ID: | 6182 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |