The MRPL10 Polyclonal Antibody (PACO06653) is an essential tool for researchers studying MRPL10, a key protein involved in mitochondrial ribosome biogenesis. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By targeting the MRPL10 protein, this antibody allows for precise detection and analysis in various cell types, making it an invaluable resource for studies in molecular biology and mitochondrial function.MRPL10 plays a crucial role in the assembly of mitochondrial ribosomes, essential for protein synthesis within the mitochondria.
Dysfunction in MRPL10 has been linked to various diseases, including mitochondrial disorders and cancer. Understanding the function and regulation of MRPL10 is essential for uncovering its role in these diseases and developing targeted therapies. The MRPL10 Polyclonal Antibody is a reliable tool for researchers investigating the intricate mechanisms of mitochondrial ribosome biogenesis and its implications in health and disease.
Antibody Name:
MRPL10 Antibody (PACO06653)
Antibody SKU:
PACO06653
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:20000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from the C-terminal region of human MRP-L10.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of 293T lysis using MRP-L10 antibody.
Western Blot analysis of COLO205 cells using MRP-L10 Polyclonal Antibody.
Synonyms:
MRPL10; MRPL8; RPML8; 39S ribosomal protein L10; mitochondrial; L10mt; MRP-L10; 39S ribosomal protein L8, mitochondrial; L8mt; MRP-L8
UniProt Protein Function:
MRPL10: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]Protein type: Ribosomal; Mitochondrial; TranslationChromosomal Location of Human Ortholog: 17q21.32Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; ribonucleoprotein complex; mitochondrial large ribosomal subunitMolecular Function: structural constituent of ribosomeBiological Process: mitochondrial translation; translation; organelle organization and biogenesis; ribosome biogenesis and assembly
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]
