The MRPL1 Polyclonal Antibody (CAB4947) is a valuable tool for researchers studying MRPL1, a mitochondrial ribosomal protein essential for protein synthesis within the mitochondria. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the MRPL1 protein, enabling accurate detection and analysis in a variety of cell types.MRPL1 is a key player in mitochondrial function, participating in the assembly of the mitochondrial ribosome and translation of mitochondrial-encoded proteins. Dysregulation of MRPL1 has been linked to mitochondrial dysfunction, which is implicated in various diseases including cancer, neurodegenerative disorders, and metabolic conditions.
By studying the role of MRPL1, researchers can gain insights into the molecular mechanisms underlying these pathologies and potentially identify new therapeutic targets.Overall, the MRPL1 Polyclonal Antibody is an essential tool for investigating the function of MRPL1 in cellular processes and disease states. Its high specificity and sensitivity make it a reliable choice for immunology, oncology, and mitochondrial biology research.
Product Name:
MRPL1 Rabbit Polyclonal Antibody
SKU:
CAB4947
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 51-325 of human MRPL1 (NP_064621.3).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family.
Purification Method:
Affinity purification
Gene ID:
65008
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MRPL1 antibody (CAB4947) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
