The MRGPRX2 Polyclonal Antibody (PACO38746) is a valuable tool for researchers studying MRGPRX2, a G protein-coupled receptor that plays a role in mediating inflammation and pain. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.MRGPRX2 is known for its involvement in mast cell activation and allergic responses, making it a target of interest in studies related to allergic diseases, inflammation, and pain pathways. The MRGPRX2 Polyclonal Antibody binds specifically to the MRGPRX2 receptor, allowing for precise detection and analysis in different cell types and tissues.
By understanding the mechanisms by which MRGPRX2 functions, researchers can gain insights into the development of new therapeutic approaches for conditions such as allergies, chronic pain, and inflammatory disorders. The MRGPRX2 Polyclonal Antibody is a valuable tool for investigating the role of MRGPRX2 in these pathological processes and may lead to the discovery of novel treatment strategies.
Western blot. All lanes: MRGPRX2 antibody at 5µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Mouse liver tissue. Lane 3: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 38 kDa. Observed band size: 38 kDa.
Immunofluorescent analysis of MCF-7 cells using PACO38746 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction. Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.
Synonyms:
Mas-related G-protein coupled receptor member X2, MRGPRX2, MRGX2
UniProt Protein Function:
MRGPRX2: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin- stimulated cAMP levels, suggesting that this receptor is G(q)- coupled. Belongs to the G-protein coupled receptor 1 family. Mas subfamily.Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1Chromosomal Location of Human Ortholog: 11p15.1Cellular Component: integral to membrane; integral to plasma membraneMolecular Function: G-protein coupled receptor activity; neuropeptide bindingBiological Process: mast cell activation; mast cell degranulation; positive regulation of cytokinesis
