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Mpox (Monkeypox) Virus (MPXV) A29 ELISA Kit (MKRS0001)

SKU:
MKRS0001
Product Type:
ELISA Kit
Size:
96 Assays
ELISA Type:
Sandwich
Reactivity:
Human
€829
Frequently bought together:

Description

Product Name:Mpox (Monkeypox) Virus (MPXV) A29 ELISA Kit
Product Code:MKRS0001
Species Detected:Virus
Gene Names:A29L
Protein Name & Synonyms:A29
Specifity:This ELISA antibody pair detects MPXV A29.
Sensitivity:6.55 pg/ml
Detection Range:6.55 pg/ml - 20 ng/ml
Recommended Dilution (Serum & Plasma):3 fold
Compatiable Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich
Number of Targets Detected:1
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Solid Support:96-well Microplate
Size:96T
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other Materials Required

  1. Distilled or deionized water
  2. Precision pipettes to deliver 2 µl to 1 µl volumes
  3. Adjustable 1-25 µl pipettes for reagent preparation
  4. 100 µl and 1 liter graduated cylinders
  5. Tubes to prepare standard and sample dilutions
  6. Absorbent paper
  7. Microplate reader capable of measuring absorbance at 450nm
  8. Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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