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Mpox (Monkeypox) Virus A35R Protein Human IgA ELISA Kit (MKRS0011)

SKU:
MKRS0011
Product Type:
ELISA Kit
Size:
96 Assays
ELISA Type:
Indirect
Reactivity:
Human
€599
Frequently bought together:

Description

Monkeypox Virus A35R Protein Human IgA ELISA Kit

The Monkeypox Virus A35R Protein Human IgA ELISA Kit is a specialized diagnostic tool designed for the accurate detection of IgA antibodies specific to the A35R protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying Monkeypox virus infection and immunity. The A35R protein is a key antigen involved in the immune response to Monkeypox virus infection, making this kit an essential tool for understanding the host immune response to this pathogen.

By detecting IgA antibodies against the A35R protein, researchers can gain valuable insights into the immune status of individuals exposed to Monkeypox virus and track the progression of infection.This Monkeypox Virus A35R Protein Human IgA ELISA Kit is ideal for a wide range of research applications, including epidemiological studies, vaccine development, and therapeutic research. With its high level of accuracy and precision, this kit is a valuable asset for researchers working in the field of infectious diseases and virology.

Product Name:Mpox (Monkeypox) Virus A35R Protein Human IgA ELISA Kit
Product Code:MKRS0011
Species Detected:Human
Gene Symbols:IGA
Compatible Sample Types:Plasma, Serum
Design Principle:Indirect
Number of Targets Detected:1
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Solid Support:96-well Microplate
Size:96T
  1. Pre-coated 96-well microplate
  2. Wash Buffer
  3. Positive Control
  4. Biotinylated Detection Antibody
  5. Biotinylated Anti-Positive Control
  6. HRP-Streptavidin concentrate
  7. TMB One-Step Substrate
  8. Stop Solution
  9. Assay Diluent
  10. 5X Sample Diluent

Other Materials Required

  1. Microplate reader capable of measuring absorbance at 450 nm
  2. Precision pipettes to deliver 2 µl to 1 ml volumes
  3. Adjustable 1-25 ml pipettes for reagent preparation
  4. 100 ml and 1 liter graduated cylinders
  5. Absorbent paper
  6. Distilled or deionized water
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
  3. Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
  4. Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
  5. Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
  6. Add 50 µl Stop Solution to each well. Read at 450 nm immediately.

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