The MPL Polyclonal Antibody (CAB16715) is a valuable tool for researchers studying the MPL protein, a key regulator of hematopoiesis and immune response. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the MPL protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in immunology and cancer research.The MPL protein, also known as myeloproliferative leukemia protein, plays a crucial role in the development and function of blood cells, making it a promising target for studying hematological disorders and cancer.
Understanding the mechanisms by which MPL regulates hematopoiesis and immune response could lead to the development of novel therapies for diseases such as leukemia, thrombocytopenia, and immune-related disorders. The MPL Polyclonal Antibody is a valuable tool for researchers seeking to expand their knowledge of MPL biology and its implications in disease progression.
Product Name:
TPO-R/CD110/c-Mpl Rabbit Polyclonal Antibody
SKU:
CAB16715
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 295-390 of human TPO-R/CD110/c-Mpl (NP_005364.1).
cell surface, external side of plasma membrane, Golgi apparatus, nuclear membrane, plasma membrane
Calculated MW:
71kDa
Observed MW:
Refer to figures
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Purification Method:
Affinity purification
Gene ID:
4352
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using TPO-R/CD110/c-Mpl Rabbit pAb (CAB16715) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
