The MOV10L1 Polyclonal Antibody (PAC059401) is developed for researchers interested in studying MOV10L1, a protein known for its involvement in RNA processing and gene regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting MOV10L1 in human samples, making it ideal for use in a variety of research applications such as Western blotting.MOV10L1 is a member of the Mov10 family of proteins, which are known to play critical roles in RNA silencing and post-transcriptional gene regulation. MOV10L1 has been implicated in various cellular processes, including mRNA stability and transportation, as well as viral restriction.
By targeting MOV10L1 with this antibody, researchers can gain valuable insights into its functions and potential implications in diseases such as cancer and viral infections.Understanding the role of MOV10L1 in RNA regulation and gene expression is essential for advancing our knowledge of cellular processes and developing targeted therapies for diseases associated with dysregulated gene expression. The MOV10L1 Polyclonal Antibody provides a valuable tool for researchers seeking to investigate the functions of MOV10L1 in different biological contexts.
Western Blot. Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate. All lanes: MOV10L1 antibody at 6.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 136, 38, 14, 130, 131 kDa. Observed band size: 136 kDa.
IHC image of PACO59401 diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO59401 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the primary piRNA metabolic process. Specifically binds to piRNA precursors and promotes the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Acts via its ATP-dependent RNA helicase activity: displays 5'-3' RNA unwinding activity and probably mediates unwinding and funneling of single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins.
Synonyms:
RNA helicase Mov10l1 (EC 3.6.4.13) (Moloney leukemia virus 10-like protein 1) (MOV10-like protein 1), MOV10L1
UniProt Protein Function:
MOV10L1: Putative RNA helicase. Isoform 1 may play a role in male germ cell development. Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Helicase; EC 3.6.4.13Chromosomal Location of Human Ortholog: 22q13.33Cellular Component: intracellularMolecular Function: RNA binding; magnesium ion binding; ATP binding; ATP-dependent RNA helicase activityBiological Process: multicellular organismal development; spermatogenesis; gene expression; germ cell development
UniProt Protein Details:
NCBI Summary:
This gene is similar to a mouse gene that encodes a putative RNA helicase and shows testis-specific expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
