The Mouse IL-12 ELISA Kit is a specialized assay designed for the precise quantification of Interleukin-12 (IL-12) levels in a variety of biological samples. IL-12, a crucial cytokine with potent immunoregulatory functions, plays a pivotal role in modulating the cellular activities that drive immune responses. Accurate measurement of IL-12 is essential for understanding its function in enhancing cell-mediated immunity, regulating T cell responses, and influencing various inflammatory processes. The detection and quantification of IL-12 can provide valuable insights into its role in immune system modulation and its implications in various inflammatory diseases and infections.
Employing high sensitivity and specificity, the Mouse IL-12 ELISA Kit from Assay Genie ensures precise and reproducible results. Manufactured under strict quality control measures, this ELISA kit guarantees robust performance and ease of use, making it an excellent choice for research applications aimed at elucidating the intricate functions of IL-12 in immunology and inflammatory responses.
Product Name:
MouseIL-12 ELISA Kit
SKU:
AEES00251
Target:
MouseIL-12
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.17 pg/mL
Detection range:
0.31-20 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-12. You can calculate the concentration of Mouse IL-12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
85-96
96-108
Average (%)
91
91
102
1:4
Range (%)
82-98
86-101
98-109
Average (%)
89
93
104
1:8
Range (%)
85-98
85-95
95-108
Average (%)
92
90
101
1:16
Range (%)
87-97
87-100
92-105
Average (%)
92
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
90
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
95-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.48
6.82
0.91
2.37
6.4
Standard deviation
0.06
0.13
0.35
0.05
0.12
0.33
C V (%)
6.48
5.39
5.08
5.61
4.97
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-12 in samples. No significant cross-reactivity or interference between Mouse IL-12 and analogues was observed.
