Mouse Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) ELISA Kit
The Mouse Zona Pellucida Glycoprotein 2 (Sperm Receptor ZP2) ELISA Kit is specifically designed for the accurate quantification of ZP2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Zona pellucida glycoproteins are essential for the sperm-egg interaction during fertilization, with ZP2 playing a critical role as a sperm receptor. Understanding the levels of ZP2 can provide valuable insights into reproductive biology and fertility studies.
This ELISA kit is an indispensable tool for researchers studying fertilization mechanisms and developing potential interventions for infertility.Easy to use and versatile, the Mouse Zona Pellucida Glycoprotein 2 ELISA Kit from Assay Genie is a reliable solution for researchers looking to accurately measure ZP2 levels in mouse samples. Unlock the potential of your research with this cutting-edge ELISA kit.
Product Name:
Mouse Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) ELISA Kit
Product Code:
MODL01408
Size:
96 Assays
Target:
ZP2
Synonyms:
ZPA, Zona Pellucida Sperm-Binding Protein 2 , Zona pellucida protein A
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
