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Mouse X-Ray Repair Cross Complementing 6 (XRCC6) ELISA Kit

SKU:
MODL01405
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P23475
Sensitivity:
0.107ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Ku70,CTC75,CTCBF,G22P1,ML8,TLAA,Lupus Ku Autoantigen Protein P70,ATP-Dependent DNA Helicase 2 Subunit 1,CTC box-binding factor 75 kDa,Thyroid-lupus autoantigen
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse X-Ray Repair Cross Complementing 6 (XRCC6) ELISA Kit

Based on the information provided in the URL, the Mouse X-Ray Repair Cross Complementing 6 (XRCC6) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of XRCC6 levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an ideal tool for various research applications.XRCC6 is a critical protein involved in DNA repair processes, particularly in response to DNA damage caused by ionizing radiation. Understanding the expression levels of XRCC6 can provide valuable insights into the mechanisms of DNA repair and their implications in conditions such as cancer, radiation therapy, and genetic disorders.

By utilizing the Mouse XRCC6 ELISA Kit, researchers can effectively study the role of XRCC6 in maintaining genomic stability and its potential as a therapeutic target for certain disease conditions. This kit is a valuable resource for laboratories conducting research in the fields of molecular biology, genetics, and oncology.

Product Name:Mouse X-Ray Repair Cross Complementing 6 (XRCC6) ELISA Kit
Product Code:MODL01405
Size:96 Assays
Target:XRCC6
Synonyms:Ku70, CTC75, CTCBF, G22P1, ML8, TLAA, Lupus Ku Autoantigen Protein P70, ATP-Dependent DNA Helicase 2 Subunit 1, CTC box-binding factor 75 kDa, Thyroid-lupus autoantigen
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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