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Mouse Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit

SKU:
MODL01394
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8BH43
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SCAR2,WAVE2,Verprolin homology domain-containing protein 2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit

The Mouse WASF2 ELISA Kit is specially designed for the precise measurement of the Wiskott-Aldrich Syndrome Protein Family Member 2 (WASF2) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for researchers working on various biological studies.WASF2 is a key protein involved in cell motility and cytoskeleton organization, playing a critical role in processes such as cell migration and immune response.

Dysregulation of WASF2 has been linked to disorders like cancer, autoimmune diseases, and neurological disorders, making it a valuable biomarker for understanding disease mechanisms and developing potential treatments. With its advanced technology and reliable performance, the Mouse WASF2 ELISA Kit is an essential tool for researchers seeking to investigate the role of WASF2 in physiological and pathological conditions, paving the way for new insights and therapeutic interventions.

Product Name:Mouse Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit
Product Code:MODL01394
Size:96 Assays
Target:WASF2
Synonyms:SCAR2, WAVE2, Verprolin homology domain-containing protein 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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