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Mouse Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) ELISA Kit

SKU:
MODL01368
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q7TMF5
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SERPINA12,OL-64,Visceral Adipose-Specific SERPIN,Serine Proteinase Inhibitor,Clade A Member 12,Visceral adipose-specific serpin
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) ELISA Kit

The Mouse Visceral Adipose Tissue-derived Serine Protease Inhibitor (Vaspin) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of Vaspin levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Vaspin is an important serine protease inhibitor derived from visceral adipose tissue, playing a significant role in metabolic disorders such as obesity and diabetes.

It is also involved in inflammation and insulin sensitivity, making it a valuable biomarker for studying these conditions and potential therapeutic targets.Overall, the Mouse Vaspin ELISA Kit is a powerful tool for researchers looking to investigate the role of Vaspin in metabolic disorders and inflammation, providing precise and reliable data for their studies.

Product Name:Mouse Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) ELISA Kit
Product Code:MODL01368
Size:96 Assays
Target:Vaspin
Synonyms:SERPINA12, OL-64, Visceral Adipose-Specific SERPIN, Serine Proteinase Inhibitor,Clade A Member 12, Visceral adipose-specific serpin
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)86-10093
EDTA plasma (n=5)87-10194
Heparin plasma (n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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