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Mouse VEGF-B (Vascular Endothelial cell Growth Factor B) CLIA Kit (MOES00589)

SKU:
MOES00589
Product Type:
ELISA Kit
ELISA Type:
CLIA Kit
Size:
96 Assays
Sensitivity:
4.69pg/mL
Range:
7.81-500pg/mL
ELISA Type:
Sandwich
Synonyms:
VEGFB, VEGFL, VRF
Reactivity:
Mouse
Sample Type:
Serum, plasma and other biological fluids
Research Area:
Cardiovascular
€649
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Mouse VEGF-B (Vascular Endothelial cell Growth Factor B) CLIA Kit
SKU:MOES00589
Target:Mouse VEGF-B (Vascular Endothelial cell Growth Factor B)
Size:96T
Assay type:Sandwich-CLIA
Assay time:3.5h
Sensitivity:4.69 pg/mL
Detection range:7.81-500 pg/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
CLIA Plate8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent A1 vial, 5 mL1 vial, 5 mL4°C (shading light)
Substrate Reagent B1 vial, 5 mL1 vial, 5 mL
Desiccant11
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VEGF-B. You can calculate the concentration of Mouse VEGF-B in the samples by comparing the RLU value of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)91-10894-108100-113
Average (%)99102107
1:4Range (%)100-11493-106104-119
Average (%)10898110
1:8Range (%)98-112101-11399-111
Average (%)105106105
1:16Range (%)89-10390-10492-103
Average (%)959897
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)86-9991
EDTA plasma (n=5)89-10196
Cell culture media (n=5)89-10496
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n20.020.020.020.020.020.0
Mean (ng/mL)24.6544.93195.3126.0248.09186.17
Standard deviation2.954.4516.192.225.3317.05
C V (%)11.979.98.298.5311.089.16
Sample type &Sample volume:serum, plasma and other biological fluids; 100μL
Reproducibility:Both intra-CV and inter-CV are < 15%.
Application:This CLIA kit applies to the in vitro quantitative determination of Mouse VEGF-B concentrations in serum, plasma and other biological fluids.
Specificity:This kit recognizes Mouse VEGF-B in samples. No significant cross-reactivity or interference between Mouse VEGF-B and analogues was observed.

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