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Mouse Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit

SKU:
MODL01378
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35969
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Flt1,Fms-Related Tyrosine Kinase 1,Vascular Permeability Factor Receptor,Fms-like tyrosine kinase 1,Tyrosine-protein kinase receptor FLT
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€599
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Description

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Mouse Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit

The Mouse Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit is a high-quality assay designed for the precise measurement of VEGFR1 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VEGFR1 is a key receptor involved in angiogenesis, playing a critical role in the regulation of blood vessel formation and maintenance. Dysregulation of VEGFR1 has been linked to various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable target for therapeutic interventions.

By utilizing the Mouse VEGFR1 ELISA Kit, researchers can gain valuable insights into the role of VEGFR1 in disease progression and treatment response. With its user-friendly protocol and reliable performance, this kit is an essential tool for studying angiogenesis and developing novel therapeutic strategies.

Product Name:Mouse Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit
Product Code:MODL01378
Size:96 Assays
Target:VEGFR1
Synonyms:Flt1, Fms-Related Tyrosine Kinase 1, Vascular Permeability Factor Receptor, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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