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Mouse Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

SKU:
MODL01369
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29533
Sensitivity:
0.23ng/mL
Range:
0.39-25ng/mL
ELISA Type:
Sandwich
Synonyms:
CD106,INCAM-100,L1CAM
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Mouse Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

The Mouse VCAM-1 (Vascular Cell Adhesion Molecule-1) ELISA Kit is a high-quality and reliable assay kit designed for the quantification of VCAM-1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VCAM-1 is a critical adhesion molecule involved in immune response regulation and endothelial cell activation, playing a key role in the inflammatory process.

Dysregulation of VCAM-1 has been linked to various diseases, including atherosclerosis, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapies.With this Mouse VCAM-1 ELISA Kit, researchers can effectively measure VCAM-1 levels in mouse samples, enabling a better understanding of its role in disease pathogenesis and providing valuable insights for targeted therapeutic interventions.

Product Name:Mouse Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
Product Code:MODL01369
Size:96 Assays
Target:VCAM1
Synonyms:CD106, INCAM-100, L1CAM
Detection Method:Sandwich
Range:0.39-25ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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