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Mouse V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) ELISA Kit

SKU:
MODL00434
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27577
Sensitivity:
0.042ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
EWSR2,p54,Protein C-ets-1
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Mouse V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) ELISA Kit

The Mouse ETS1 ELISA Kit offered by Assay Genie is a cutting-edge tool for precise measurement of the ETS1 protein levels in mouse samples. This kit delivers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which are essential for various research purposes.ETS1, also known as v-ets erythroblastosis virus E26 oncogene homolog 1, is a key transcription factor involved in regulating gene expression and cell proliferation. Its functions are crucial in various physiological and pathological processes, including development, immunity, and cancer.

Monitoring ETS1 levels can provide valuable insights into disease mechanisms and potential therapeutic interventions.In summary, the Mouse ETS1 ELISA Kit from Assay Genie is a reliable and efficient tool for researchers studying the role of ETS1 in various biological processes and diseases. Its high performance and ease of use make it a valuable addition to any laboratory conducting ETS1-related research.

Product Name:Mouse V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) ELISA Kit
Product Code:MODL00434
Size:96 Assays
Target:ETS1
Synonyms:EWSR2, p54, Protein C-ets-1
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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