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Mouse Urocortin 3 (UCN3) ELISA Kit

SKU:
MODL01357
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q924A4
Sensitivity:
4.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
SCP,SPC,UCNIII,Stresscopin
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Urocortin 3 (UCN3) ELISA Kit

The Mouse Urocortin-3 (UCN3) ELISA Kit is a powerful tool for the accurate measurement of UCN3 levels in mouse serum, plasma, and tissue lysates. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.UCN3 is a key regulatory peptide involved in stress responses, cardiovascular function, and energy homeostasis. Its dysregulation has been implicated in conditions such as anxiety disorders, heart disease, and metabolic disorders, making it a valuable biomarker for studying these diseases and exploring potential treatment options.

Overall, the Mouse UCN3 ELISA Kit offers researchers a reliable and efficient method for quantifying UCN3 levels in mouse samples, facilitating in-depth investigations into its physiological and pathological roles.

Product Name:Mouse Urocortin 3 (UCN3) ELISA Kit
Product Code:MODL01357
Size:96 Assays
Target:UCN3
Synonyms:SCP, SPC, UCNIII, Stresscopin
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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