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Mouse UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit

SKU:
MODL01359
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88693
Sensitivity:
0.28ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
GCS,GLCT-1,Glucosylceramide Synthase,N-acylsphingosine D-glucosyltransferase
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit

The Mouse UDG (UDP-Glucose Ceramide Glucosyltransferase) ELISA Kit is specifically designed for the accurate measurement of UDG levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.UDG is an important enzyme involved in the biosynthesis of glycosphingolipids, playing a critical role in cellular processes such as cell growth, differentiation, and apoptosis.

Dysregulation of UDG has been linked to various diseases including cancer, metabolic disorders, and autoimmune diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its user-friendly protocol and reliable performance, the Mouse UDG ELISA Kit is an essential tool for researchers looking to delve into the mechanisms underlying UDG function and its implications in disease pathology.

Product Name:Mouse UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit
Product Code:MODL01359
Size:96 Assays
Target:UGCG
Synonyms:GCS, GLCT-1, Glucosylceramide Synthase, N-acylsphingosine D-glucosyltransferase
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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