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Mouse Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit

SKU:
MODL01353
Product Type:
ELISA Kit
Sensitivity:
0.107ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ANCR,AS,E6-AP,EPVE6AP,HPVE6A,Papilloma Virus E6-Associated,Angelman Syndrome,Oncogenic protein-associated protein E6-AP,Renal carcinoma antigen NY-REN-54
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit

The Mouse Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit is specifically designed for the quantitative detection of UBE3A levels in mouse samples including serum, plasma, and cell lysates. With excellent sensitivity and specificity, this kit provides accurate and reliable results for your research needs.UBE3A is an important E3 ubiquitin ligase enzyme that plays a key role in protein degradation and regulation. Dysregulation of UBE3A has been linked to various diseases such as Angelman syndrome, autism, and cancer, highlighting its significance as a potential therapeutic target.

This ELISA kit is essential for studying the function and expression levels of UBE3A in various biological processes and disease conditions. Its easy-to-use format and precise quantification make it a valuable tool for researchers seeking to understand the role of UBE3A in cellular pathways and disease mechanisms.

Product Name:Mouse Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit
Product Code:MODL01353
Size:96 Assays
Target:UBE3A
Synonyms:ANCR, AS, E6-AP, EPVE6AP, HPVE6A, Papilloma Virus E6-Associated,Angelman Syndrome, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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