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Mouse Tumor Protein, Translationally Controlled 1 (TPT1) ELISA Kit

SKU:
MODL01328
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P63028
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HRF,TCTP,p02,p23,Fortilin,Translationally-Controlled Tumor Protein,Histamine-releasing factor
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Tumor Protein, Translationally Controlled 1 (TPT1) ELISA Kit

The Mouse Tumor Protein Translationally Controlled 1 (TPT1) ELISA Kit is specifically designed for the accurate quantification of TPT1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an indispensable tool for various research applications.TPT1 is a protein known to be involved in tumor progression and cell proliferation. It plays a crucial role in cancer development and has been identified as a potential therapeutic target.

By measuring TPT1 levels, researchers can gain valuable insights into tumor growth and progression, as well as potentially identifying new treatment strategies for cancer.Overall, the Mouse TPT1 ELISA Kit offers a powerful tool for studying the role of TPT1 in cancer and other diseases, providing researchers with valuable data to advance their understanding of this important protein.

Product Name:Mouse Tumor Protein, Translationally Controlled 1 (TPT1) ELISA Kit
Product Code:MODL01328
Size:96 Assays
Target:TPT1
Synonyms:HRF, TCTP, p02, p23, Fortilin, Translationally-Controlled Tumor Protein, Histamine-releasing factor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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