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Mouse Tumor Protein P63 (TP63) ELISA Kit

SKU:
MODL01317
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88898
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
P63,TP73L,CUSP,EEC3,KET,LMS,OFC8,RHS,SHFM4,TP53CP,Tumor Protein p53-Competing Protein,Chronic ulcerative stomatitis protein,Keratinocyte transcription factor
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Tumor Protein P63 (TP63) ELISA Kit

The Mouse Tumor Protein p63 (TP63) ELISA Kit is specifically developed for the precise measurement of TP63 levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees dependable and consistent results, making it a valuable tool for various research studies.TP63, also known as tumor protein 63, is a key player in cell growth, differentiation, and apoptosis regulation.

It is implicated in the development and progression of numerous cancers, including squamous cell carcinomas and other malignancies. By enabling accurate quantification of TP63 levels, this ELISA kit is essential for investigating the role of TP63 in cancer biology and potential therapeutic interventions.

Product Name:Mouse Tumor Protein P63 (TP63) ELISA Kit
Product Code:MODL01317
Size:96 Assays
Target:TP63
Synonyms:P63, TP73L, CUSP, EEC3, KET, LMS, OFC8, RHS, SHFM4, TP53CP, Tumor Protein p53-Competing Protein, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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