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Mouse Tumor Protein p53 (TP53) ELISA Kit

SKU:
MODL01316
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02340
Sensitivity:
2.46pg/mL
Range:
6.25-400pg/mL
ELISA Type:
Sandwich
Synonyms:
p53,LFS1,TRP53,Li-Fraumeni Syndrome,Cellular tumor antigen p53,Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Mouse Tumor Protein p53 (TP53) ELISA Kit

The Mouse Tumor Protein p53 (TP53) ELISA Kit is specifically designed for the precise measurement of TP53 levels in mouse samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable tool for various research applications.TP53, also known as tumor protein p53, is a key tumor suppressor gene that regulates cell growth and prevents the formation of tumors.

Abnormalities in TP53 have been linked to various cancers, making it a crucial biomarker for studying cancer progression and developing new therapeutic strategies.By using the Mouse Tumor Protein p53 (TP53) ELISA Kit, researchers can gain valuable insights into the role of TP53 in cancer development and progression, ultimately leading to advancements in cancer diagnosis and treatment.

Product Name:Mouse Tumor Protein p53 (TP53) ELISA Kit
Product Code:MODL01316
Size:96 Assays
Target:TP53
Synonyms:p53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Detection Method:Sandwich
Range:6.25-400pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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