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Mouse Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) ELISA Kit

SKU:
MODL01304
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27512
Sensitivity:
5.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD40,CDW40,Bp50,P50,B-cell surface antigen CD40,CD40L receptor
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Mouse Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) ELISA Kit

The Mouse Tumor Necrosis Factor Receptor Superfamily Member 5 (TNFRSF5) ELISA Kit is specifically designed to detect TNFRSF5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TNFRSF5 plays a crucial role in regulating immune responses and promoting cell survival. Dysregulation of TNFRSF5 has been associated with a variety of autoimmune diseases, inflammatory disorders, and cancer.

By accurately measuring TNFRSF5 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic interventions.Overall, the Mouse TNFRSF5 ELISA Kit is an essential tool for studying the role of TNFRSF5 in various physiological and pathological processes, offering reliable and precise measurements for advanced research studies.

Product Name:Mouse Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) ELISA Kit
Product Code:MODL01304
Size:96 Assays
Target:TNFRSF5
Synonyms:CD40, CDW40, Bp50, P50, B-cell surface antigen CD40, CD40L receptor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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