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Mouse Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit

SKU:
MODL01303
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P47741
Sensitivity:
13.7pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD134,TNFRSF4,ACT35,OX40,TXGP1L,TAX transcriptionally-activated glycoprotein 1 receptor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit

The Mouse Tumor Necrosis Factor Receptor Superfamily Member 4 (TNFRSF4) ELISA Kit is specifically designed for the accurate detection of TNFRSF4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TNFRSF4 is a key receptor involved in regulating immune responses and inflammation, making it a valuable target for studying autoimmune diseases, cancer, and other inflammatory conditions.

By accurately measuring TNFRSF4 levels, researchers can gain insight into the role of this receptor in disease progression and potentially identify new therapeutic strategies.Overall, the Mouse TNFRSF4 ELISA Kit provides researchers with a reliable tool for studying the function of TNFRSF4 in various disease contexts and advancing our understanding of immune regulation and inflammation in mouse models.

Product Name:Mouse Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit
Product Code:MODL01303
Size:96 Assays
Target:TNFRSF4
Synonyms:CD134, TNFRSF4, ACT35, OX40, TXGP1L, TAX transcriptionally-activated glycoprotein 1 receptor
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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