null

Mouse Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17) ELISA Kit

SKU:
MODL01302
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88472
Sensitivity:
0.101ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD269,BCM,BCMA,B-cell maturation protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17) ELISA Kit

The Mouse Tumor Necrosis Factor Receptor Superfamily Member 17 (TNFRSF17) ELISA Kit is a powerful tool for accurate measurement of TNFRSF17 levels in mouse samples such as serum, plasma, and tissue homogenates. This kit is known for its high sensitivity and specificity, ensuring precise and reliable results for a variety of research purposes.TNFRSF17, also known as BCMA (B cell maturation antigen), plays a crucial role in regulating B cell survival and function. Dysregulation of TNFRSF17 has been implicated in various diseases such as autoimmune disorders, lymphomas, and multiple myeloma.

Therefore, this ELISA kit is essential for studying the role of TNFRSF17 in these conditions and for developing potential therapeutic interventions.Overall, the Mouse TNFRSF17 ELISA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of TNFRSF17 in disease pathology and for developing targeted therapies to modulate its activity.

Product Name:Mouse Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17) ELISA Kit
Product Code:MODL01302
Size:96 Assays
Target:TNFRSF17
Synonyms:CD269, BCM, BCMA, B-cell maturation protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products