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Mouse Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

SKU:
MODL01299
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06804
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
DIF,TNF-A,TNFSF2,Cachectin,Tumor Necrosis Factor Ligand Superfamily Member 2,TNF,Tumor Necrosis Factor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Mouse Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

The Mouse Tumor Necrosis Factor Alpha (TNFa) ELISA Kit is a powerful tool for quantifying levels of TNFa in mouse serum, plasma, and tissue lysate samples. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in a variety of research applications.TNFa is a key cytokine involved in inflammation and immune response, playing a crucial role in various physiological and pathological processes. Dysregulation of TNFa has been linked to a variety of diseases, including autoimmune disorders, inflammatory conditions, and cancer.

Thus, the Mouse TNFa ELISA Kit is essential for studying the role of TNFa in these conditions and exploring potential therapeutic interventions.With its user-friendly protocol and robust performance, the Mouse TNFa ELISA Kit is a valuable tool for researchers seeking to advance their understanding of TNFa biology and its implications for health and disease.

Product Name:Mouse Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
Product Code:MODL01299
Size:96 Assays
Target:TNFa
Synonyms:DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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