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Mouse Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit

SKU:
MODL01300
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O08859
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TSG6,TNF-Stimulated Gene 6,Hyaluronate-binding protein,Tumor necrosis factor alpha-induced protein 6
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit

The Mouse TNFAIP6 (Tumor Necrosis Factor Alpha-Induced Protein 6) ELISA Kit is a powerful tool for the quantitative measurement of TNFAIP6 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for a variety of research applications.TNFAIP6 is a key protein involved in the inflammatory response, playing a crucial role in conditions such as arthritis, atherosclerosis, and cancer.

By detecting TNFAIP6 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Whether investigating the pathogenesis of inflammatory disorders or exploring novel treatment strategies, the Mouse TNFAIP6 ELISA Kit is an essential tool for researchers seeking to advance our understanding of TNFAIP6 biology.

Product Name:Mouse Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit
Product Code:MODL01300
Size:96 Assays
Target:TNFaIP6
Synonyms:TSG6, TNF-Stimulated Gene 6, Hyaluronate-binding protein, Tumor necrosis factor alpha-induced protein 6
Detection Method:Sandwich
Range:0.156-10ng/mL.
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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