Mouse Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit
The Mouse Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit is a valuable tool for researchers studying the role of TPPP in various biological processes. This kit is specifically designed for the accurate detection of TPPP levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it suitable for a wide range of research applications.TPPP is known to play a crucial role in promoting tubulin polymerization, a process essential for cell division and various cellular functions.
Its dysregulation has been linked to neurodegenerative disorders, making it a potential target for therapeutic interventions. By accurately measuring TPPP levels, researchers can gain valuable insights into its role in disease pathogenesis and potential treatment strategies.Overall, the Mouse TPPP ELISA Kit is a valuable tool for researchers seeking to better understand the role of TPPP in biological processes and disease mechanisms. Its high sensitivity and specificity make it a reliable choice for obtaining accurate and reproducible data in research studies.
Product Name:
Mouse Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit
Product Code:
MODL01325
Size:
96 Assays
Target:
TPPP
Synonyms:
TPPP1, p25alpha, p25, TPPP/p25, Brain Specific Protein p25 Alpha, 25 kDa brain-specific protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
