null

Mouse Tropomyosin 2 Beta (TPM2) ELISA Kit (MODL01322)

SKU:
MODL01322
Product Type:
ELISA Kit
Sensitivity:
0.278ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
AMCD1,DA1,TMSB,Arthrogryposis Multiplex Congenital,Distal,Type 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649

Description

system_update_altDatasheet

Mouse Tropomyosin 2 Beta (TPM2) ELISA Kit

The Mouse Tropomyosin 2 Beta (TPM2) ELISA Kit is specifically designed for the quantitative measurement of TPM2 levels in mouse serum, plasma, and tissue homogenates. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TPM2 is a key protein involved in regulating muscle contraction and plays a critical role in maintaining muscle structure and function.

Dysregulation of TPM2 has been implicated in various muscle disorders, making it an important biomarker for studying muscle-related diseases and potential therapeutic interventions.With its reliable performance and precise measurements, the Mouse TPM2 ELISA Kit is an essential tool for researchers investigating the role of TPM2 in muscle biology and disease pathogenesis.

Product Name:Mouse Tropomyosin 2 Beta (TPM2) ELISA Kit
Product Code:MODL01322
Size:96 Assays
Target:TPM2
Synonyms:AMCD1, DA1, TMSB, Arthrogryposis Multiplex Congenital,Distal,Type 1
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products