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Mouse Tripeptidyl Peptidase I (TPP1) ELISA Kit

SKU:
MODL01324
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O89023
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CLN2,GIG1,LPIC,Tripeptidyl aminopeptidase,Lysosomal pepstatin-insensitive protease,Ceroid-Lipofuscinosis,Neuronal 2,Late Infantile,Cell growth-inhibiting gene 1 protein
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Tripeptidyl Peptidase I (TPP1) ELISA Kit

The Mouse Tripeptidyl Peptidase I (TPP1) ELISA Kit is a powerful tool for accurate measurement of TPP1 levels in mouse serum, plasma, and cell culture samples. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.TPP1 is an important enzyme involved in the degradation of proteins within lysosomes, playing a key role in maintaining cellular homeostasis. Dysregulation of TPP1 activity has been linked to various diseases including lysosomal storage disorders and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.

With easy-to-follow protocols and rapid assay times, the Mouse TPP1 ELISA Kit is a valuable resource for researchers studying lysosomal function, neurodegeneration, and other related fields. Upgrade your research with this innovative ELISA kit from Assay Genie.

Product Name:Mouse Tripeptidyl Peptidase I (TPP1) ELISA Kit
Product Code:MODL01324
Size:96 Assays
Target:TPP1
Synonyms:CLN2, GIG1, LPIC, Tripeptidyl aminopeptidase, Lysosomal pepstatin-insensitive protease, Ceroid-Lipofuscinosis,Neuronal 2,Late Infantile, Cell growth-inhibiting gene 1 protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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