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Mouse Trehalase (TREH) ELISA Kit

SKU:
MODL01330
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JLT2
Sensitivity:
0.216ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
TRE,TREA,Brush Border Membrane Glycoprotein,Alpha,Alpha-Trehalase,Alpha,Alpha-Trehalose Glucohydrolase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Trehalase (TREH) ELISA Kit

The Mouse Trehalase (TREH) ELISA Kit is specifically designed for the quantitative detection of trehalase levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.Trehalase is an important enzyme that plays a key role in carbohydrate metabolism, particularly in the breakdown of trehalose. Dysregulation of trehalase activity has been implicated in various metabolic disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

Overall, the Mouse Trehalase ELISA Kit is a valuable tool for researchers looking to investigate the role of trehalase in mouse physiology and disease pathology. Its ease of use and highly precise measurements make it a must-have for any laboratory studying carbohydrate metabolism and related disorders.

Product Name:Mouse Trehalase (TREH) ELISA Kit
Product Code:MODL01330
Size:96 Assays
Target:TREH
Synonyms:TRE, TREA, Brush Border Membrane Glycoprotein, Alpha,Alpha-Trehalase, Alpha,Alpha-Trehalose Glucohydrolase
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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