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Mouse Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

SKU:
MODL01261
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q62395
Sensitivity:
29.14pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
HITF,ITF3,TFI,HP1.B
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

Hi there! Introducing our Mouse Trefoil Factor 3 (Intestinal TFF3) ELISA Kit from Assay Genie. This kit is specifically designed for the accurate detection of mouse TFF3 levels in serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for various research applications.TFF3 is a critical protein involved in maintaining the integrity of the mucosal barrier in the gastrointestinal tract.

It plays a crucial role in protecting and repairing the epithelial lining of the intestines, making it an essential biomarker for studying conditions such as inflammatory bowel disease, gastrointestinal cancers, and other intestinal disorders.Don't miss out on the opportunity to enhance your research with our Mouse TFF3 ELISA Kit. Order yours today and unlock new insights into intestinal health and disease.

Product Name:Mouse Trefoil Factor 3, Intestinal (TFF3) ELISA Kit
Product Code:MODL01261
Size:96 Assays
Target:TFF3
Synonyms:HITF, ITF3, TFI, HP1.B
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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