null

Mouse Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit

SKU:
MODL01339
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q704Y3
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
VR1,OTRPC1,Vanilloid Receptor Subtype 1,Capsaicin receptor,Osm-9-like TRP channel 1,Vanilloid receptor 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit

The Mouse TRPV1 (Transient Receptor Potential Cation Channel Subfamily V Member 1) ELISA Kit is specifically designed for the precise measurement of TRPV1 levels in mouse serum, plasma, and tissue samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TRPV1 is a key protein involved in sensory and pain signaling, making it a valuable target for studying pain perception and developing novel pain treatment strategies.

Additionally, TRPV1 has been implicated in various pathological conditions such as inflammatory pain, neuropathic pain, and migraine, highlighting its importance as a potential therapeutic target.Overall, the Mouse TRPV1 ELISA Kit provides researchers with a reliable tool for studying the role of TRPV1 in physiological and pathological processes, paving the way for future advancements in pain management and drug development.

Product Name:Mouse Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit
Product Code:MODL01339
Size:96 Assays
Target:TRPV1
Synonyms:VR1, OTRPC1, Vanilloid Receptor Subtype 1, Capsaicin receptor, Osm-9-like TRP channel 1, Vanilloid receptor 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products