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Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7) ELISA Kit (MODL01338)

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SKU:
MODL01338
Product Type:
ELISA Kit
Sensitivity:
34pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CHAK,CHAK1,LTRPC7,TRP-PLIK,Channel-kinase 1,Long transient receptor potential channel 7
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649

Description

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Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7) ELISA Kit

The Mouse TRPM7 (Transient Receptor Potential Cation Channel Subfamily M Member 7) ELISA Kit is specifically designed for the accurate measurement of TRPM7 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TRPM7 is a critical protein involved in ion transport and cellular signaling pathways, playing a key role in various physiological processes.

Dysregulation of TRPM7 has been linked to numerous diseases, including cardiovascular disorders, neurological conditions, and cancer, making it a valuable biomarker for disease research and potential therapeutic interventions.Overall, the Mouse TRPM7 ELISA Kit provides researchers with a powerful tool for studying the role of TRPM7 in health and disease, offering precise and accurate measurements to advance scientific understanding in this field.

Product Name:Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7) ELISA Kit
Product Code:MODL01338
Size:96 Assays
Target:TRPM7
Synonyms:CHAK, CHAK1, LTRPC7, TRP-PLIK, Channel-kinase 1, Long transient receptor potential channel 7
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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