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Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit

SKU:
MODL01336
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q7TN37
Sensitivity:
0.048ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TRPM4B,Melastatin-4,Calcium-activated non-selective cation channel 1,Long transient receptor potential channel 4
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit

The Mouse Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit is specifically designed for the accurate quantification of TRPM4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TRPM4 is a key ion channel that regulates calcium signaling and is involved in various physiological processes such as cardiac conduction, smooth muscle contraction, and insulin secretion.

Dysregulation of TRPM4 has been linked to diseases like cardiac arrhythmias, hypertension, and diabetes, making it a valuable target for further research and potential therapeutic interventions.With the Mouse TRPM4 ELISA Kit, researchers can gain valuable insights into the role of TRPM4 in health and disease, paving the way for new treatment strategies and advancements in the field of ion channel biology.

Product Name:Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit
Product Code:MODL01336
Size:96 Assays
Target:TRPM4
Synonyms:TRPM4B, Melastatin-4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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