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Mouse Transforming Growth Factor Beta Receptor II (TGFbR2) ELISA Kit

SKU:
MODL01272
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q62312
Sensitivity:
0.105ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MFS2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Mouse Transforming Growth Factor Beta Receptor II (TGFbR2) ELISA Kit

The Mouse Transforming Growth Factor Beta Receptor II (TGFBR2) ELISA Kit is specifically designed for the accurate quantification of TGFBR2 levels in mouse serum, plasma, and tissue culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TGFBR2 is a key receptor involved in the TGF-beta signaling pathway, playing a critical role in cell growth, differentiation, and immune response regulation. Dysregulation of TGFBR2 has been implicated in various diseases, including cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

With this ELISA kit, researchers can confidently measure TGFBR2 levels in mouse samples, allowing for a deeper understanding of its role in disease pathology and potential for targeted treatment strategies. Don't miss out on this essential tool for your research endeavors.

Product Name:Mouse Transforming Growth Factor Beta Receptor II (TGFbR2) ELISA Kit
Product Code:MODL01272
Size:96 Assays
Target:TGFbR2
Synonyms:MFS2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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