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Mouse Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit

SKU:
MODL01270
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P82198
Sensitivity:
22.5pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-BI,CSD3,LCD1,CSD1,CSD2,BIGH3,CDB1,CDGG1,RGD-CAP,Kerato-Epithelin,RGD-Containing Collagen-Associated Protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit

The Mouse Transforming Growth Factor Beta-Induced Protein (TGFBI) ELISA Kit is a powerful tool for the precise measurement of TGFBI levels in mouse serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it the perfect choice for a variety of research applications.TGFBI is a key player in the regulation of cell adhesion, migration, and proliferation, making it a crucial factor in various biological processes.

Its dysregulation has been linked to numerous diseases, including cancer, fibrosis, and neurodegenerative disorders, underscoring its significance as a potential diagnostic and therapeutic target.Investigate TGFBI dynamics with confidence using the Mouse TGFBI ELISA Kit, and uncover valuable insights into its role in health and disease.

Product Name:Mouse Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit
Product Code:MODL01270
Size:96 Assays
Target:TGFbI
Synonyms:TGF-BI, CSD3, LCD1, CSD1, CSD2, BIGH3, CDB1, CDGG1, RGD-CAP, Kerato-Epithelin, RGD-Containing Collagen-Associated Protein
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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