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Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

SKU:
MODL01269
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3TRQ9
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B3,LAP,Latency-associated peptide
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

The Mouse Transforming Growth Factor Beta 3 (TGFβ3) ELISA Kit is a reliable tool for detecting TGFβ3 levels in mouse serum, plasma, and cell culture supernatants with high sensitivity and specificity. This kit provides accurate and reproducible results, making it ideal for various research applications.Transforming Growth Factor Beta 3 is a crucial regulator of cell growth, differentiation, and development. It plays a significant role in various biological processes such as embryogenesis, wound healing, and immune response.

Dysregulation of TGFβ3 is associated with diseases like cancer, fibrosis, and developmental disorders, making it an important biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse Transforming Growth Factor Beta 3 (TGFβ3) ELISA Kit is a valuable tool for researchers seeking to explore the role of TGFβ3 in biological processes and diseases in mouse models.

Product Name:Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit
Product Code:MODL01269
Size:96 Assays
Target:TGFb3
Synonyms:TGF-B3, LAP, Latency-associated peptide
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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