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Mouse Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

SKU:
MODL01268
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27090
Sensitivity:
13.2pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B2,TGF-Beta2,G-TSF,LAP,Cetermin,Polyergin,Latency-associated peptide,BSC-1 cell growth inhibitor,Glioblastoma-derived T-cell suppressor factor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

The Mouse Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of TGFb2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying the role of TGFb2 in various biological processes.Transforming Growth Factor Beta 2 (TGFb2) is a key regulator of cell growth, differentiation, and apoptosis. It plays a critical role in numerous physiological and pathological processes, including immune response, wound healing, and cancer progression.

By accurately measuring TGFb2 levels, researchers can gain valuable insights into the mechanisms underlying these processes and identify potential therapeutic targets.Overall, the Mouse TGFb2 ELISA Kit is a valuable tool for researchers seeking to better understand the role of TGFb2 in various biological processes and diseases, providing accurate and reliable data for their studies.

Product Name:Mouse Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit
Product Code:MODL01268
Size:96 Assays
Target:TGFb2
Synonyms:TGF-B2, TGF-Beta2, G-TSF, LAP, Cetermin, Polyergin, Latency-associated peptide, BSC-1 cell growth inhibitor, Glioblastoma-derived T-cell suppressor factor
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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