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Mouse Transforming Growth Factor Alpha (TGFa) ELISA Kit

SKU:
MODL01266
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P48030
Sensitivity:
5.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ETGF,TGF-A,Protransforming Growth Factor alpha,EGF-Like TGF
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Transforming Growth Factor Alpha (TGFa) ELISA Kit

The Mouse Transforming Growth Factor Alpha (TGFA) ELISA Kit from Assay Genie is a powerful tool for the detection of TGFA levels in mouse serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for a wide range of research applications.TGFA is a key growth factor involved in cell growth, development, and tissue repair. It plays a crucial role in various biological processes, including embryogenesis, wound healing, and cancer progression.

Studying TGFA levels can provide valuable insights into these processes and help in the development of new therapeutic strategies.The Assay Genie Mouse TGFA ELISA Kit offers a user-friendly protocol and efficient assay performance, making it a valuable tool for researchers looking to investigate the role of TGFA in different disease models and biological pathways. Order your kit today and unlock the potential of TGFA research in mouse models.

Product Name:Mouse Transforming Growth Factor Alpha (TGFa) ELISA Kit
Product Code:MODL01266
Size:96 Assays
Target:TGFa
Synonyms:ETGF, TGF-A, Protransforming Growth Factor alpha, EGF-Like TGF
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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