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Mouse Transferrin Receptor 2 (TFR2) ELISA Kit (MODL01264)

SKU:
MODL01264
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JKX3
Sensitivity:
0.61ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
HFE3,TFRC2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Transferrin Receptor 2 (TFR2) ELISA Kit

The Mouse Transferrin Receptor 2 (TFR2) ELISA Kit is a reliable and accurate tool for detecting TFR2 levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.TFR2 is a vital protein involved in iron metabolism and plays a crucial role in regulating iron levels in the body.

Dysregulation of TFR2 has been linked to various disorders, including hemochromatosis and other iron-related conditions, making it an important biomarker for studying these diseases and potential treatment options.With its exceptional performance and ease of use, the Mouse Transferrin Receptor 2 ELISA Kit is a valuable tool for researchers looking to investigate the role of TFR2 in health and disease.

Product Name:Mouse Transferrin Receptor 2 (TFR2) ELISA Kit
Product Code:MODL01264
Size:96 Assays
Target:TFR2
Synonyms:HFE3, TFRC2
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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