The Mouse TPP (Thrombus Precursor Protein) CLIA Kit is a reliable and accurate tool for detecting levels of TPP in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and reproducible results for various research purposes.TPP, also known as thrombus precursor protein, is a key player in the formation of blood clots and thrombosis. Understanding TPP levels can provide valuable insights into thrombosis-related conditions such as heart attacks, strokes, and deep vein thrombosis.
This kit is essential for studying the role of TPP in these conditions and exploring potential therapeutic interventions.Overall, the Mouse TPP CLIA Kit from AssayGenie is a valuable resource for researchers looking to investigate the role of TPP in thrombosis and related disorders, offering accurate and reliable measurements for a wide range of applications.
Product Name:
Mouse TpP (Thrombus Precursor Protein) CLIA Kit
SKU:
MOES00560
Target:
Mouse TpP (Thrombus Precursor Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TpP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TpP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TpP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TpP. You can calculate the concentration of Mouse TpP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
96-106
99-110
Average (%)
94
101
104
1:4
Range (%)
95-108
100-113
91-105
Average (%)
103
105
97
1:8
Range (%)
90-102
91-102
87-100
Average (%)
97
96
94
1:16
Range (%)
96-113
85-96
96-113
Average (%)
103
91
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-115
106
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
409.87
908.16
2931.58
412.12
845.11
2717.08
Standard deviation
35.95
66.57
199.64
50.94
90.76
274.15
C V (%)
8.77
7.33
6.81
12.36
10.74
10.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TpP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TpP in samples. No significant cross-reactivity or interference between Mouse TpP and analogues was observed.
