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Mouse Toll Like Receptor 3 (TLR3) ELISA Kit

SKU:
MODL01291
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q99MB1
Sensitivity:
23.64pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD283
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Toll Like Receptor 3 (TLR3) ELISA Kit

The Mouse Toll-Like Receptor 3 (TLR3) ELISA Kit is a valuable tool for the precise quantification of TLR3 levels in mouse samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research applications.TLR3 is a key component of the innate immune system, playing a vital role in recognizing and responding to viral infections.

By measuring TLR3 levels, researchers can gain valuable insights into the immune response and develop strategies for combating viral diseases and developing novel therapies.Overall, the Mouse Toll-Like Receptor 3 (TLR3) ELISA Kit is a reliable and efficient solution for studying immune responses and advancing research in the field of immunology.

Product Name:Mouse Toll Like Receptor 3 (TLR3) ELISA Kit
Product Code:MODL01291
Size:96 Assays
Target:TLR3
Synonyms:CD283
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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