The Mouse Tenascin C (TNC) ELISA Kit is an advanced assay designed to quantitatively measure the levels of Tenascin C, an essential extracellular matrix protein, in various biological samples with high accuracy and sensitivity.
Tenascin C is a versatile glycoprotein involved in a wide range of biological processes, including cell adhesion, migration, tissue development, and wound healing. Its expression is often associated with tissue remodeling, cancer progression, and inflammatory responses, making it a valuable target for research in various fields. By precisely detecting and quantifying TNC levels, researchers can gain valuable insights into its role in modulating cell behavior, immune responses, and disease pathogenesis. Understanding the intricate functions of Tenascin C is crucial for unraveling its impact on tissue homeostasis and disease progression, offering potential therapeutic avenues for conditions influenced by alterations in TNC expression. The Mouse TNC ELISA Kit by Assay Genie provides exceptional sensitivity and specificity, ensuring accurate and reproducible results for your experimental studies. Manufactured under strict quality control conditions, this kit delivers reliable performance and user-friendly protocols, making it a reliable tool for researchers exploring the roles of Tenascin C in various biological contexts. Choose Assay Genie's TNC ELISA Kit to unlock the mysteries of Tenascin C biology and pave the way for novel discoveries in tissue biology, cancer research, and regenerative medicine applications.
Product Name:
Mouse TNC (Tenascin C) ELISA Kit
SKU:
AEES00240
Target:
Mouse TNC (Tenascin C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNC. You can calculate the concentration of Mouse TNC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-106
90-97
85-95
Average (%)
99
94
90
1:4
Range (%)
93-103
92-102
88-99
Average (%)
96
95
93
1:8
Range (%)
93-106
87-97
96-105
Average (%)
99
91
99
1:16
Range (%)
95-106
102-113
89-98
Average (%)
101
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-103
97
EDTA plasma (n=5)
85-95
91
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.74
230.98
978.91
89.83
227.4
914.52
Standard deviation
5.54
10.49
51.49
4.57
10.67
44.08
C V (%)
5.85
4.54
5.26
5.09
4.69
4.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TNC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNC in samples. No significant cross-reactivity or interference between Mouse TNC and analogues was observed.
