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Mouse Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

SKU:
MODL01284
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P12032
Sensitivity:
27pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
EPO,CLGI,EPA,HCI,Erythroid Potentiating Activity,Collagenase Inhibitor,Metalloproteinase inhibitor 1,Fibroblast collagenase inhibitor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Mouse Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

The Mouse TIMP1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is specially designed to accurately quantify TIMP1 levels in mouse tissue samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.TIMP1 is a key regulator of extracellular matrix remodeling and tissue homeostasis, making it a crucial biomarker in studies related to inflammation, fibrosis, and cancer progression.

Understanding TIMP1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its reliable performance and easy-to-use format, the Mouse TIMP1 ELISA Kit is an essential tool for researchers investigating the role of TIMP1 in various physiological and pathological processes in mouse models.

Product Name:Mouse Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit
Product Code:MODL01284
Size:96 Assays
Target:TIMP1
Synonyms:EPO, CLGI, EPA, HCI, Erythroid Potentiating Activity, Collagenase Inhibitor, Metalloproteinase inhibitor 1, Fibroblast collagenase inhibitor
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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