null

Mouse Tissue factor / Coagulation Factor III ELISA Kit

SKU:
MOFI01143
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P20352
Sensitivity:
3.75pg/ml
Range:
6.25-400pg/ml
ELISA Type:
Sandwich
Synonyms:
TF, Tissue factor, F3, FIII, CD142, TFA, Thromboplastin, Coagulation Factor III
Reactivity:
Mouse
Research Area:
Cardiovascular
€649
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Mouse Tissue factor/Coagulation Factor III ELISA Kit

The Mouse Tissue Factor (Coagulation Factor III) ELISA Kit is specifically designed for the accurate quantification of tissue factor levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results for various research applications.Tissue factor, also known as coagulation factor III, is a critical protein involved in the initiation of the coagulation cascade pathway. It plays a crucial role in blood clotting and thrombosis, making it a valuable biomarker for studying various thrombotic disorders and cardiovascular diseases.

By accurately measuring tissue factor levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially develop novel therapeutic strategies.Overall, the Mouse Tissue Factor (Coagulation Factor III) ELISA Kit is a reliable tool for studying tissue factor biology and its implications in various disease states, offering researchers a robust and efficient method for analyzing tissue factor levels in mouse samples.

Product Name:Mouse Tissue factor / Coagulation Factor III ELISA Kit
Product Code:MOFI01143
Size:96 Assays
Alias:TF, Tissue factor, F3, FIII, CD142, TFA, Thromboplastin, Coagulation Factor III
Detection Method:Sandwich ELISA
Application:This immunoassay kit allows for the in vitro quantitative determination of Mouse TF concentrations in serum plasma and other biological fluids.
Sensitivity:3.75pg/ml
Range:6.25-400pg/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery: Matrices listed below were spiked with certain level of Mouse TF and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse TF in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)87-10194
EDTA plasma(n=5)88-10196
UFH plasma(n=5)86-10194
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse TF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-92%85-105%86-101%
EDTA plasma(n=5)85-95%84-99%86-100%
UFH plasma(n=5)82-96%81-97%85-98%
Intra Assay:CV <8%
Inter Assay:CV <10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP20352
UniProt Protein Function:F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, integral

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular space; integral to membrane; membrane; plasma membrane

Molecular Function:hematopoietin/interferon-class (D200-domain) cytokine receptor activity; phospholipid binding; protease binding

Biological Process: activation of plasma proteins during acute inflammatory response; blood coagulation; caspase activation; cytokine and chemokine mediated signaling pathway; hemostasis; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell migration

NCBI Summary:This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]
UniProt Code:P20352
NCBI GenInfo Identifier:135667
NCBI Gene ID:14066
NCBI Accession:P20352.2
UniProt Related Accession:P20352
Molecular Weight:32,935 Da
NCBI Full Name:Tissue factor
NCBI Synonym Full Names:coagulation factor III
NCBI Official Symbol:F3  
NCBI Official Synonym Symbols:TF; Cf3; Cf-3; CD142; AA409063  
NCBI Protein Information:tissue factor
UniProt Protein Name:Tissue factor
UniProt Synonym Protein Names:Coagulation factor III; CD_antigen: CD142
Protein Family:Tissue factor
UniProt Gene Name:F3  
UniProt Entry Name:TF_MOUSE

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3. Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4. Add 0.1 ml of properly diluted sample (Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6. Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7. Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9. Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10. Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11. Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12. Add 90 µL of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13. Add 50 µL of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum:

If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

Plasma:

Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.

Urine & Cerebrospinal Fluid:

Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.

Cell culture supernatant:

Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.

Cell lysates:

Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20°C.

Tissue homogenates:

The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.

Tissue lysates:

Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.

Breast Milk:

Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

Related Products